pcr_results
| Name | PCR_Results.txt |
| JSON File Name | PCR_Results.json |
| Type | multiple |
| Schema Version | 3.37 |
| Description | The qRT-PCR experiment sample template defines and annotates the assay results for a sample by linking sample, experiment, and results together. More than one analyte's results per assayed sample may be reported by copying at least the group of columns 'Entrez Gene ID' and 'Threshold Cycles(ct)' needed to describe each assay result. |
| Download | ⬇️ PCR_Results.xlsx ⬇️ PCR_Results.txt ⬇️ PCR_Results.json This top-level schema references companion files (e.g. PCR_Results.Datum.json) in the same json-templates folder — download the all_templates.zip to get them all together. |
Reading this table: see How to read the template documentation for what Required / Conditionally required / Optional mean and how to read the Type & Constraints column.
| Template Column | Requirement | Type & Constraints | Description |
|---|---|---|---|
| Expsample ID | Required | Text, max 15 characters Reference — must already exist (in this package or the workspace) as a expsampleJSON: expsampleId (string) | The experiment sample identifier must be stored in ImmPort or in the experimentsamples.txt template. Please enter either an experiment sample user defined ID or ImmPort accession. |
| Gene Symbol Name | Required | Preferred vocabulary — reported value is kept; a preferred term from lk_analyte is filled in when it matches JSON: geneSymbolName (enum) | The NCBI Gene symbol for the gene being assayed. Please select a gene symbol from the list provided if the gene symbol matches your symbol or enter a symbol if there is not an appropriate one provided. This symbol is visible when the result is shared. If the gene symbol is a NCBI Gene Symbol that is provided in the list, then the columns 'Gene Name' and 'Gene ID' will also be overwritten by the gene name and Entrez Gene ID provided by NCBI. |
| Value Reported | Required | Text, max 50 characters JSON: valueReported (number) | This value could be absolute or relative. For example, an absolute expression value could be 6 ng RNA/mg intestine. In this case, 6 should be entered in the 'Expression value of target RNA' column, while ng RNA/ mg intestine is in the 'Expression unit of target RNA' column. A relative expression value, like signal versus GAPDH, could be 2.07. In this case, 2.07 should be in the 'Expression value of target RNA' column, while relative to GAPDH is in the 'Expression unit of target RNA' column. A number is expected. |
| Unit Reported | Required | Text, max 200 characters Preferred vocabulary — reported value is kept; a preferred term from lk_pcr_expression_unit is filled in when it matches JSON: unitReported (enum) | The unit for the Expression Value Of Target Nucleic ACID. Please select a unit from the list provided if the unit matches your unit or enter a unit if there is not an appropriate one provided. |
| Gene ID | Optional | Text, max 10 characters JSON: geneId (string) | The NCBI Gene ID for the gene being assayed. A number is expected. |
| Gene Name | Optional | Text, max 4000 characters JSON: geneName (string) | The NCBI Gene name for the gene being assayed. |
| Other Gene Accession | Optional | Text, max 250 characters JSON: otherGeneAccession (string) | Additional identifier(s) for the gene being assayed. |
| Comments | Optional | Text, max 500 characters JSON: comments (string) | Comments captures additional descriptive information. |