DR27 DataRelease

Release Date: 07/03/2018

SDY1260: Correlation between human innate and adaptive immune responses to T-cell independent or dependent meningococcal vaccines.
Status:	New
Description:	Thirty healthy adults (18-45 years of age) with no history of prior meningococcal vaccination and no contraindications to immunization will be vaccinated using either the meningococcal conjugate vaccine (MCV) or the meningococcal non-conjugate polysaccharide vaccine (MPSV). The study will be conducted as a randomized double blinded trial. Vaccine administration will be performed by an unblinded vaccine administrator, who will not be involved in subsequent assessments. Blood samples will be collected on Days D0 (at enrollment) and D3, D7, D14, D30 and D180 post vaccination to study innate and adaptive immunity markers. Even though meningococcal vaccination is considered safe, volunteers are asked to report any local or systemic AEs for 30 days post vaccination and any SAEs for 180 days post vaccination.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Biological Analysis of Innate and Adaptive Responses to Vaccination
DOI:	10.21430/M3F47KSLLP
Subjects:	30
Study PI, contact:	Name Organization Site Bali Pulendran Emory University Emory Hope Clnic
Publications:	Molecular signatures of antibody responses derived from a systems biology study of five human vaccines.. Nat Immunol. Feb 2014. doi: 10.1038/ni.2789. Epub 2013 Dec 15. [Pubmed: 24336226]
Resources:	GEO https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52245]
Assays:	Assay Type Number of Exp. Samples ELISA 711 Transcription profiling by array 90
Clinical Assessments:	None

SDY1264: Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans
Status:	New
Description:	A major challenge in vaccinology is to prospectively determine vaccine efficacy. Here we have used a systems biology approach to identify early gene signatures that predicted immune responses in humans vaccinated with yellow fever vaccine YF-17D. Vaccination induced genes that regulate virus innate sensing and type I interferon production. Computational analyses identified a gene signature, including complement protein C1qB and eukaryotic translation initiation factor 2 alpha kinase 4 an orchestrator of the integrated stress response that correlated with and predicted YF-17D CD8+ T cell responses with up to 90% accuracy in an independent, blinded trial. A distinct signature, including B cell growth factor TNFRS17, predicted the neutralizing antibody response with up to 100% accuracy. These data highlight the utility of systems biology approaches in predicting vaccine efficacy.
Program/Contract:	Program Contract Other Programs Sanofi Pasteur
DOI:	10.21430/M3XTBR8F18
Subjects:	25
Study PI, contact:	Name Organization Site Bali Pulendran Emory University Emory Hope Clinic
Publications:	Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans.. Nat Immunol. Jan 2009. doi: 10.1038/ni.1688. Epub 2008 Nov 23. [Pubmed: 19029902]
Resources:	GEO https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13485]
Assays:	Assay Type Number of Exp. Samples Transcription profiling by array 87 Virus Neutralization 25
Clinical Assessments:	None

SDY1276: Time series of global gene expression after trivalent influenza vaccination in humans
Status:	New
Description:	The relationship between gene expression patterns and humoral immune response to vaccination was analyzed in order to understand the interindividual variability among immune reponse to vaccination
Program/Contract:	Program Contract NIAID Program Viral Respiratory Pathogens Research Unit (VRPRU)-266030039
DOI:	10.21430/M3J92GN8I3
Subjects:	226
Study PI, contact:	Name Organization Site John Belmont Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States Baylor College of Medicine
Publications:	Early patterns of gene expression correlate with the humoral immune response to influenza vaccination in humans.. J Infect Dis. Apr 2011. doi: 10.1093/infdis/jiq156. Epub 2011 Feb 28. [Pubmed: 21357945] Integrative genomic analysis of the human immune response to influenza vaccination.. Elife. Jul 2013. doi: 10.7554/eLife.00299. [Pubmed: 23878721]
Resources:	Time series of global gene expression after trivalent influenza vaccination in humans (male cohort) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48018] Time series of global gene expression after trivalent influenza vaccination in humans (female cohort) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE480238] Time series of global gene expression after trivalent influenza vaccination in humans https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48024]
Assays:	Assay Type Number of Exp. Samples Hemagglutination Inhibition 2034 Neutralizing Antibody Titer Assay 2034 Transcription profiling by array 848
Clinical Assessments:	None

SDY1289: YFV integrated mulitlineage and polyfunctional responses
Status:	New
Description:	Correlates of immune-mediated protection to most viral and cancer vaccines are still unknown. This impedes the development of novel vaccines to incurable diseases such as HIV and cancer. In this study, we have used functional genomics and polychromatic flow cytometry to define the signature of the immune response to the yellow fever (YF) vaccine 17D (YF17D) in a cohort of 40 volunteers followed for up to 1 yr after vaccination. We show that immunization with YF17D leads to an integrated immune response that includes several effector arms of innate immunity, including complement, the inflammasome, and interferons, as well as adaptive immunity as shown by an early T cell response followed by a brisk and variable B cell response. Development of these responses is preceded, as demonstrated in three independent vaccination trials and in a novel in vitro system of primary immune responses (modular immune in vitro construct [MIMIC] system), by the coordinated up-regulation of transcripts for specific transcription factors, including STAT1, IRF7, and ETS2, which are upstream of the different effector arms of the immune response. These results clearly show that the immune response to a strong vaccine is preceded by coordinated induction of master transcription factors that lead to the development of a broad, polyfunctional, and persistent immune response that integrates all effector cells of the immune system. peripheral blood samples from human newborns
Program/Contract:	Program Contract Other Programs Canadian Network for Vaccines and Immunotherapeutics (CANVAC)
DOI:	10.21430/M37CO9E6FQ
Subjects:	30
Study PI, contact:	Name Organization Site Rafick-Pierre Sekaly McGill University Department of Microbiology and Immunology, McGill University
Publications:	Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses.. J Exp Med December 2008. doi: 10.1084/jem.20082292 [Pubmed: 19047440]
Resources:	Publication http://jem.rupress.org/content/jem/205/13/3119.full.pdf?with-ds=yes] NCBI GEO https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13699]
Assays:	Assay Type Number of Exp. Samples Neutralizing Antibody Titer Assay 160 Transcription profiling by array 142
Clinical Assessments:	None

SDY1293: Expression of genes associated with immunoproteasome processing of major histocompatibility complex peptides is indicative of protection with adjuvanted RTS,S malaria vaccine.
Status:	New
Description:	Background. Patterns of expressed genes in the peripheral blood mononuclear cells of persons who were receiving RTS,S/AS01 or RTS,S/AS02 malaria vaccine and were undergoing experimental challenge with mosquito-borne falciparum malaria were examined to identify markers associated with protection.
Program/Contract:	Program Contract Other Programs Walter Reed Army Institute of Research
DOI:	10.21430/M3ETOL8TGS
Subjects:	58
Study PI, contact:	Name Organization Site Christian Ockenhouse Walter Reed Army Institute of Research Malaria Research Initiative
Publications:	Expression of genes associated with immunoproteasome processing of major histocompatibility complex peptides is indicative of protection with adjuvanted RTS,S malaria vaccine.. Journal of Infectious Diseases February 2010. doi: https://doi.org/10.1086/650310 [Pubmed: 20078211]
Resources:	Publication https://doi.org/10.1086/650310] NCBI GEO https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18323]
Assays:	Assay Type Number of Exp. Samples Transcription profiling by array 254
Clinical Assessments:	None

SDY1299: Identification of Three Rheumatoid Arthritis Disease Subtypes By Machine Learning Integration of Synovial Histologic Features and RNA Sequencing Data
Status:	New
Description:	Gene expression analysis of RA and OA synovial tissue revealed 3 distinct synovial subtypes. These labels were used to generate a histologic scoring algorithm in which the histologic scores were found to be associated with parameters of systemic inflammation, including the erythrocyte sedimentation rate, CRP levels, and autoantibody levels. Comparison of gene expression patterns to clinical features revealed a potentially clinically important distinction: mechanisms of pain may differ in patients with different synovial subtypes.
Program/Contract:	Program Contract Accelerating Medicines Partnership (AMP) Accelerating Medicines Partnership (AMP)
DOI:	10.21430/M3V2G6PBYS
Subjects:	45
Study PI, contact:	Name Organization Site Laura Donlin Hospital for Special Surgery, The Rockefeller University Hospital for Special Surgery
Publications:	Identification of Three Rheumatoid Arthritis Disease Subtypes by Machine Learning Integration of Synovial Histologic Features and RNA Sequencing Data.. Arthritis Rheumatol. May 2018. doi: 10.1002/art.40428. Epub 2018 Apr 2. [Pubmed: 29468833]
Resources:	Hospital for Special Surgery www.hss.edu] Accelerating Medicines Partnership Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE)? https://www.nih.gov/research-training/accelerating-medicines-partnership-amp/autoimmune-diseases-rheumatoid-arthritis-lupus]
Assays:	Assay Type Number of Exp. Samples Array 45 RNA sequencing 45
Clinical Assessments:	Arthritis Evaluation

SDY1328: Transcriptional profiling of HBV-naive subjects before vaccination against Hepatitis A/B viruses, Diphtheria/Tetanus toxoids and Cholera.
Status:	New
Description:	Mechanisms of poor responses to vaccines remain unknown. Hepatitis B virus-naive elderly subjects received three vaccines, including a vaccine against hepatitis B virus (HBV). Pre-vaccination high dimensional analyses of blood using transcriptional profiling and flow cytometry revealed that subjects having increased memory B cell frequencies and higher expression of genes downstream of B cell receptor signaling responded more strongly to the HBV vaccine whereas subjects having higher expression of inflammatory related genes and greater numbers of activated innate immune cells showed a weaker response to this vaccine. The heme-induced response was associated with the poor response to the hepatitis B vaccine. Transcriptional profiling and flow cytometry results were validated in a distinct set of elderly subjects with accuracy greater than 60%. Our study is the first that identifies baseline predictors of responses to vaccines in a population of subjects known to be highly susceptible to infections.
Program/Contract:	Program Contract Other Programs Merck & Co. Inc
DOI:	10.21430/M3ID8ZC1AT
Subjects:	174
Study PI, contact:	Name Organization Site Rafick-Pierre Sekaly Case Western Reserve University Case Western Reserve University
Publications:	Pre-vaccination inflammation and B-cell signalling predict age-related hyporesponse to hepatitis B vaccination.. Nat Commun. Jan 2016. doi: 10.1038/ncomms10369. [Pubmed: 26742691]
Resources:	ClinicalTrials.gov NCT01119703] Gene Expression Omnibus GSE65834]
Assays:	Assay Type Number of Exp. Samples DNA microarray 169
Clinical Assessments:	None

SDY1337: Sequence-based HLA-A, B, C, DP, DQ, and DR typing
Status:	New
Description:	DNA sequence-based typing at the HLA-A, -B, -C, -DPB1, -DQA1, -DQB1, and -DRB1 loci
Program/Contract:	Program Contract Protective Immunity Following Dengue Virus Natural Infections and Vaccination Protective Immunity Following Dengue Virus Natural Infections and Vaccination
DOI:	10.21430/M3M9Y78QLF
Subjects:	339
Study PI, contact:	Name Organization Site Alessandro Sette La Jolla Institute for Allergy and Immunology La Jolla Institute for Allergy and Immunology
Publications:	Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 339 adults from Managua, Nicaragua.. Hum Immunol. Jan 2018. doi: 10.1016/j.humimm.2017.11.002. Epub 2017 Nov 7. [Pubmed: 29122684] Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 339 adults from Managua, Nicaragua. Human Immunology January 2018. doi: 10.1016/j.humimm.2017.11.002 [Pubmed: PMC5788571]
Resources:
Assays:	Assay Type Number of Exp. Samples HLA Typing 339
Clinical Assessments:	None

SDY67: Bioinformatics Approach to 2010-2011 TIV Influenza A/H1N1 Vaccine Immune Profiling
Status:	Updated
Description:	Aim 1: Characterize Immune Profiles Over Time, Aim 2: Correlate Immune Profiles with Vaccine Immunogenicity,Aim 3: Replication of Immune Profiles and Verification of Models
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Bioinformatics Approach to Influenza A/H1N1 Vaccine Immune Profiling
DOI:	10.21430/M3OYWCJHO1
Subjects:	159
Study PI, contact:	Name Organization Site Gregory Poland Mayo Clinic Mayo Clinic
Publications:	The impact of immunosenescence on humoral immune response variation after influenza A/H1N1 vaccination in older subjects.. PLoS One. Mar 2015. doi: 10.1371/journal.pone.0122282. eCollection 2015. [Pubmed: 25816015] System-Wide Associations between DNA-Methylation, Gene Expression, and Humoral Immune Response to Influenza Vaccination.. PLoS One. Mar 2016. doi: 10.1371/journal.pone.0152034. eCollection 2016. [Pubmed: 27031986] Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals.. Heliyon. May 2016. doi: 10.1016/j.heliyon.2016.e00098. eCollection 2016. [Pubmed: 27441275] Simultaneous enumeration of cancer and immune cell types from bulk tumor gene expression data.. Elife. Nov 2017. doi: 10.7554/eLife.26476. [Pubmed: 29130882]
Resources:	NIH Reporter 5U01AI089859-05 http://projectreporter.nih.gov/project_info_details.cfm?aid=8695082]
Assays:	Assay Type Number of Exp. Samples Cell Culture 556 DNA methylation profiling assay 952 ELISPOT 1113 Flow Cytometry 3387 Hemagglutination Inhibition 1272 Mass Spectrometry 61 Meso Scale Discovery ECL 1272 PCR 466 Q-PCR 159 RNA sequencing 1100 Virus Neutralization 635
Clinical Assessments:	None

SDY74: Systems Biology Approach to Analysis of 2010-11 TIV Fluzone Influenza Vaccine Response in Healthy Individuals (see companion studies SDY301, SDY296)
Status:	Updated
Description:	This study will measure the immune response to the influenza vaccine The long-term goal is to develop improved vaccines to infectious diseases such as influenza. Blood will be collected from patients at several visits before and after vaccination. The blood will be used in a series of immunological tests to measure the strength and breadth of immune response. These assays may include T cell and B cell activation assays, microarray testing, Epimax, Epigen, and flow cytometry.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3EJ72RVRG
Subjects:	12
Study PI, contact:	Name Organization Site A. Karolina Palucka Baylor Research Institute Baylor Research Institute
Publications:	Induction of ICOS+CXCR3+CXCR5+ TH cells correlates with antibody responses to influenza vaccination.. Sci Transl Med. Mar 2013. doi: 10.1126/scitranslmed.3005191. [Pubmed: 23486778]
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 60 Flow Cytometry 459
Clinical Assessments:	Vitals

SDY89: Systems Biology Analysis of the response to Licensed Hepatitis B Vaccine (Engerix-B) (see companion study SDY690)
Status:	Updated
Description:	This project will contribute to the overall vision and goals of this U19 by analyzing the role of adjuvants in the humoral response to hep B vaccination in healthy individuals.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3AYWX8NOT
Subjects:	50
Study PI, contact:	Name Organization Site Robert Coffman Dynavax Technologies Corporation Dynavax Technologies Corporation
Publications:	Demonstration of safety and enhanced seroprotection against hepatitis B with investigational HBsAg-1018 ISS vaccine compared to a licensed hepatitis B vaccine.. Vaccine. Mar 2012. doi: 10.1016/j.vaccine.2012.02.001. Epub 2012 Feb 14. [Pubmed: 22342916] Immunogenicity and safety of an investigational hepatitis B vaccine with a Toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared to a licensed hepatitis B vaccine in healthy adults 40-70 years of age.. Vaccine. Nov 2013. doi: 10.1016/j.vaccine.2013.05.068. Epub 2013 May 30. [Pubmed: 23727002] Immunogenicity of an investigational hepatitis B vaccine with a toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared with a licensed hepatitis B vaccine in subpopulations of healthy adults 18-70 years of age.. Vaccine. Jul 2015. doi: 10.1016/j.vaccine.2015.05.070. Epub 2015 Jun 9. [Pubmed: 26067185] None. None None None. doi: None [Pubmed: 29289383]
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 441 Flow Cytometry 384 Virus Neutralization 147
Clinical Assessments:	None

SDY91: Rituximab for the Treatment of Wegener's Granulomatosis and Microscopic Polyangiitis (RAVE ITN021AI)
Status:	Updated
Description:	Current conventional therapies for ANCA-associated vasculitis (AAV) are associated with high incidences of treatment failure, disease relapse, substantial toxicity, and patient morbidity and mortality. Rituximab is a monoclonal antibody used to treat non-Hodgkin's lymphoma. This study will evaluate the efficacy of rituximab with glucocorticoids in inducing disease remission in patients with severe forms of AAV (WG and MPA). The study consists of two phases: a 6-month remission induction phase, followed by a 12-month remission maintenance phase. All participants will receive at least 1 g of pulse intravenous methylprednisolone or a dose-equivalent of another glucocorticoid preparation. Depending on the participant's condition, he or she may receive up to 3 days of intravenous methylprednisolone for a total of 3 g of methylprednisolone (or a dose-equivalent). During the remission induction phase, all participants will receive oral prednisone daily (1 mg/kg/day, not to exceed 80 mg/day). Prednisone tapering will be completed by the Month 6 study visit. Participants will then be randomly assigned to one of two arms. Arm 1 participants will receive rituximab (375 mg/m^2) infusions once weekly for 4 weeks and cyclophosphamide (CYC) placebo daily for 3 to 6 months. Arm 2 participants will receive rituximab placebo infusions once weekly for 4 weeks and CYC daily for 3 to 6 months. During the remission maintenance phase, participants in Arm 1 will discontinue CYC placebo and start oral azathioprine (AZA) placebo daily until Month 18. Participants in Arm 2 will discontinue CYC and start AZA daily until Month 18. Participants who fail treatment before Month 6 will be crossed over to the other treatment arm unless there are specific contraindications. Participants in either group who reach clinical remission before they complete 6 months of therapy may switch from CYC/placebo to AZA/placebo if directed by their physicians.All participants will be followed for at least 18 months. Initially, study visits are weekly, progressing to monthly and then quarterly visits as the study proceeds. Blood collection will occur at each study visit.
Program/Contract:	Program Contract ITN: Collaborative Network for Clinical Research on Immune Tolerance Network Rituximab for the Treatment of Wegener's Granulomatosis and Microscopic Polyangiitis (RAVE), Immune Tolerance Network
DOI:	10.21430/M3TK42R0QR
Subjects:	197
Study PI, contact:	Name Organization Site Ulrich Specks Mayo Clinic Mayo Clinic John H. Stone Johns Hopkins University Johns Hopkins University
Publications:	Rituximab versus cyclophosphamide for ANCA-associated vasculitis.. N Engl J Med. Jul 2010. doi: 10.1056/NEJMoa0909905. [Pubmed: 20647199] Circulating markers of vascular injury and angiogenesis in antineutrophil cytoplasmic antibody-associated vasculitis.. Arthritis Rheum. Dec 2011. doi: 10.1002/art.30615. [Pubmed: 21953143] Circulating angiopoietin-2 as a biomarker in ANCA-associated vasculitis.. PLoS One. - 2012. doi: 10.1371/journal.pone.0030197. Epub 2012 Jan 18. [Pubmed: 22279570] Efficacy of remission-induction regimens for ANCA-associated vasculitis.. N Engl J Med. Aug 2013. doi: 10.1056/NEJMoa1213277. [Pubmed: 23902481] Pro: Should all patients with anti-neutrophil cytoplasmic antibody-associated vasculitis be primarily treated with rituximab?. Nephrol Dial Transplant. Jul 2015. doi: 10.1093/ndt/gfv217. Epub 2015 May 21. [Pubmed: 25999375] Reanalysis of the Rituximab in ANCA-Associated Vasculitis trial identifies granulocyte subsets as a novel early marker of successful treatment.. Arthritis Res Ther. Sep 2015. doi: 10.1186/s13075-015-0778-z. [Pubmed: 26387933] Design of the Rituximab in ANCA-associated Vasculitis (RAVE) Trial.. The Open Arthritis Journal Nov 2011. doi: 10.2174/1876539401104010001 [Pubmed: TBD_A]
Resources:	ImmuneTolerance.org http://www.immunetolerance.org/] ImmuneTolerance.org https://www.itntrialshare.org/] Clinicaltrials.gov http://clinicaltrials.gov/ct2/show/NCT00104299] The Open Arthritis Journal https://www.immunetolerance.org/sites/files/2011.11%20OpenArthritisJournal_Specks%20%28RAVE%20Design%29.pdf]
Assays:	Assay Type Number of Exp. Samples ELISA 1175 Flow Cytometry 1151
Clinical Assessments:	Assignment Baseline Height (cm) Baseline Weight (kg) BVAS/WG Diagnosis Flares Prednisone Taper Remission SF-36 Vasculitis Damage Index

SDY111: VZV vaccination in the elderly
Status:	Updated
Description:	Healthy adults (50+ years old) with history of varicella but no history of zoster are vaccinated with Zostavax. Blood and serum are taken prior to vaccination and at several points after. A systems biology approach will be used to identify age-related decreases in immune function and potential predictors and correlates of protection.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Vaccination and infection: indicators of immunological health and responsiveness
DOI:	10.21430/M3ODYABDL2
Subjects:	48
Study PI, contact:	Name Organization Site Jorg Goronzy Stanford Stanford
Publications:	B-cell repertoire responses to varicella-zoster vaccination in human identical twins.. Proc Natl Acad Sci U S A. Jan 2015. doi: 10.1073/pnas.1415875112. Epub 2014 Dec 22. [Pubmed: 25535378]
Resources:	clinicaltrials.gov https://clinicaltrials.gov/ct2/show/NCT01911065] GSE86332 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86332]
Assays:	Assay Type Number of Exp. Samples DNA microarray 20 ELISA 20 ELISPOT 80 HLA Typing 10 Luminex xMAP 168
Clinical Assessments:	None

SDY144: Systems Biology Approach to Study Influenza Vaccine 2011-12 in Healthy Children (see companion studies SDY364, SDY368, SDY387)
Status:	Updated
Description:	The treatment of pediatric immune system dysfunctions depends upon the basic understanding of its molecular and cellular components, as well as the inherent relationships between these components. Specifically, such knowledge requires an appreciation of B-Iymphocytes, T lymphocytes, natural killer cells and dendritic cells. Research investigating these cells and their functions necessitates the availability and acquisition of peripheral blood samples from healthy children to form control data groups against which various experimental conditions can be measured. Volunteers will be asked to donate blood samples to be used to further study the circulating dendritic cell subpopulations and establish their normal ranges. Blood samples will also be used to isolate antigen specific T lymphocytes, serve as a monocyte source and establish gene signatures. The assay results from SDY144's EXP13603, EXP11769, and EXP13604 are the same as for this study. The difference is how the floe cytometry results were analyzed in this study versus SDY144.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3ANETOJEC
Subjects:	17
Study PI, contact:	Name Organization Site Octavio Ramilo Nationwide Children's Hospital Nationwide Children's Hospital
Publications:	Differences in antibody responses between trivalent inactivated influenza vaccine and live attenuated influenza vaccine correlate with the kinetics and magnitude of interferon signaling in children.. J Infect Dis. Jul 2014. doi: 10.1093/infdis/jiu079. Epub 2014 Feb 4. [Pubmed: 24495909]
Resources:	Gene Expression Omnibus (GEO) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52005] EMBL-EBI http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52005/]
Assays:	Assay Type Number of Exp. Samples DNA microarray 64 Flow Cytometry 486 Hemagglutination Inhibition 32 Virus Neutralization 96
Clinical Assessments:	Medical History Vaccination History Vital Signs

SDY180: Systems scale interactive exploration reveals quantitative and qualitative differences in response to 2009-2010 Fluzone influenza vaccine and pneumococcal vaccine
Status:	Updated
Description:	Systems immunology approaches were employed to investigate innate and adaptive immune responses to influenza and pneumococcal vaccines. These two non-live vaccines show different magnitudes of transcriptional responses at different time points af- ter vaccination. Software solutions were developed to explore correlates of vaccine efficacy measured as antibody titers at day 28. These enabled a further dissection of transcriptional responses. Thus, the innate response, measured within hours in the peripheral blood, was dominated by an interferon transcriptional signature after influenza vaccination and by an inflammation signature after pneumo- coccal vaccination. Day 7 plasmablast responses induced by both vaccines was more pronounced after pneumococcal vaccination. Together, these results suggest that comparing global immune responses elicited by different vaccines will be critical to our understanding of the immune mechanisms underpinning successful vaccination.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3I44H8R17
Subjects:	46
Study PI, contact:	Name Organization Site A. Karolina Palucka Baylor Reasearch Institute Baylor Reasearch Institute
Publications:	Systems scale interactive exploration reveals quantitative and qualitative differences in response to influenza and pneumococcal vaccines.. Immunity. Apr 2013. doi: 10.1016/j.immuni.2012.12.008. [Pubmed: 23601689]
Resources:	Gene Expression Omnibus (GEO) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30101]
Assays:	Assay Type Number of Exp. Samples DNA microarray 542 Flow Cytometry 2208 Hemagglutination Inhibition 66 Luminex xMAP 229 Nanostring 18 Transcription profiling by array 161 Virus Neutralization 89
Clinical Assessments:	None

SDY183: Effect of age on 2008/2009 trivalent influenza vaccine response
Status:	Updated
Description:	To comprehensively compare the humoral immune response of young (20-31 years old) to older human subjects (60 to >90 years old) following vaccination with seasonal flu vaccine. We generated a peptide microarray featuring tiled peptides with sequences derived from the hemagglutinin proteins of multiple influenza strains. We probed the microarrays with pre- and post-vaccination serum from each age group. Serum antibody reactivity to the microarray peptides was quantified using fluorescently labeled anti-human secondary antibodies and a fluorescent microarray scanner.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Vaccination and infection: indicators of immunological health and responsiveness
DOI:	10.21430/M37TU3LVTU
Subjects:	76
Study PI, contact:	Name Organization Site PJ Utz Stanford University Stanford
Publications:	Apoptosis and other immune biomarkers predict influenza vaccine responsiveness.. Mol Syst Biol. Apr 2013. doi: 10.1038/msb.2013.15. [Pubmed: 23591775] Characterization of influenza vaccine immunogenicity using influenza antigen microarrays.. PLoS One. May 2013. doi: 10.1371/journal.pone.0064555. Print 2013. [Pubmed: 23734205]
Resources:	Gene Expression Omnibus (GEO) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE43446] Immune Epitope Database (IEDB) http://www.iedb.org/reference/1027229]
Assays:	Assay Type Number of Exp. Samples Protein microarray 152
Clinical Assessments:	None

SDY296: Systems Biology Approach to Analysis of 2011-12 TIV Fluzone Influenza Vaccine Response in Healthy Individuals (see companion studies SDY74, SDY301)
Status:	Updated
Description:	This project will contribute to the overall vision and goals of this U19 by analyzing the immune response to Flu vaccination in healthy individuals. The knowledge generated in this Project will be transferred to Projects 3-5 where immune effects of vaccination will be studied in patients with underlying immune system alterations.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M300RMFHZQ
Subjects:	45
Study PI, contact:	Name Organization Site A. Karolina Palucka Baylor Research Institute Baylor Research Institute
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 219 Flow Cytometry 975 Hemagglutination Inhibition 74 Nanostring 36 Sequencing 42 Virus Neutralization 74
Clinical Assessments:	Vaccination History

SDY299: Systems Biology Analysis of the response to Licensed Hepatitis B Vaccine (HEPLISAV) in Whole Blood (see companion studies SDY816 and SDY690)
Status:	Updated
Description:	This project will contribute to the overall vision and goals of this U19 by analyzing the role of adjuvants in the humoral response to hep B vaccination in healthy individuals.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M34QI37OT9
Subjects:	25
Study PI, contact:	Name Organization Site Robert Coffman Dynavax Technologies Corporation Dynavax Technologies Corporation
Publications:	Demonstration of safety and enhanced seroprotection against hepatitis B with investigational HBsAg-1018 ISS vaccine compared to a licensed hepatitis B vaccine.. Vaccine. Mar 2012. doi: 10.1016/j.vaccine.2012.02.001. Epub 2012 Feb 14. [Pubmed: 22342916] Immunogenicity and safety of an investigational hepatitis B vaccine with a Toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared to a licensed hepatitis B vaccine in healthy adults 40-70 years of age.. Vaccine. Nov 2013. doi: 10.1016/j.vaccine.2013.05.068. Epub 2013 May 30. [Pubmed: 23727002] Immunogenicity of an investigational hepatitis B vaccine with a toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared with a licensed hepatitis B vaccine in subpopulations of healthy adults 18-70 years of age.. Vaccine. Jul 2015. doi: 10.1016/j.vaccine.2015.05.070. Epub 2015 Jun 9. [Pubmed: 26067185] None. None None None. doi: None [Pubmed: 29289383]
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 225 Nanostring 75
Clinical Assessments:	None

SDY300: Healthy Human DC and monocyte subsets transcriptional regulations in response to Fluzone 2010-2011 and pneumococcal vaccinations
Status:	Updated
Description:	The described experiments in this study were designed to deconvolute the molecular signature observed in the whole blood of healthy subsets at early time points following the administration of Fluzone 2010-2011 vaccines. RNA-seq data generated from sorted purified cell populations, including mDC and monocyte subsets will permit us to reveal distinct roles that DC and monocyte subsets play in eliciting immune responses to vaccines against flu in healthy adults using system biology approaches.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3FJJ9G9ZZ
Subjects:	10
Study PI, contact:	Name Organization Site A. Karolina Palucka Baylor Research Institute Baylor Research Institute
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples Sequencing 90
Clinical Assessments:	None

SDY301: Systems Biology Approach to Analysis of 2012-13 TIV Fluzone Influenza Vaccine Response in Healthy Individuals (see companion studies SDY74, SDY296)
Status:	Updated
Description:	This project will contribute to the overall vision and goals of this U19 by analyzing the immune response to Flu vaccination in healthy individuals. The knowledge generated in this Project will be transferred to Projects 3-5 where immune effects of vaccination will be studied in patients with underlying immune system alterations.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3T0BGMGGC
Subjects:	40
Study PI, contact:	Name Organization Site A. Karolina Palucka Baylor Research Institute Baylor Research Institute
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 213 Flow Cytometry 642 Hemagglutination Inhibition 240 Nanostring 38 Sequencing 108 Virus Neutralization 240
Clinical Assessments:	Vaccination History

SDY311: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2010 (See companion studies SDY315 2012 / SDY312 2009 / SDY314 2008 / SDY112 2011)
Status:	Updated
Description:	Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Vaccination and infection: indicators of immunological health and responsiveness
DOI:	10.21430/M33MSDRJ55
Subjects:	76
Study PI, contact:	Name Organization Site Mark Davis Stanford University Stanford-LPCH Vaccine Program
Publications:	None
Resources:	clinicaltrials.gov https://clinicaltrials.gov/ct2/show/NCT01827462]
Assays:	Assay Type Number of Exp. Samples CyTOF 79 DNA microarray 73 Flow Cytometry 464 Hemagglutination Inhibition 140 Luminex xMAP 426
Clinical Assessments:	None

SDY312: T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination (TIV) - 2009 (See companion studies SDY315 2012 / SDY314 2008 / SDY311 2010 / SDY112 2011)
Status:	Updated
Description:	Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Vaccination and infection: indicators of immunological health and responsiveness
DOI:	10.21430/M3G230OYOM
Subjects:	84
Study PI, contact:	Name Organization Site Mark Davis Stanford University Stanford-LPCH Vaccine Program
Publications:	Defective Signaling in the JAK-STAT Pathway Tracks with Chronic Inflammation and Cardiovascular Risk in Aging Humans.. Cell Syst. Oct 2016. doi: 10.1016/j.cels.2016.09.009. Epub 2016 Oct 13. [Pubmed: 27746093]
Resources:	clinicaltrials.gov https://clinicaltrials.gov/ct2/show/NCT01827462]
Assays:	Assay Type Number of Exp. Samples DNA microarray 73 Flow Cytometry 1155 Hemagglutination Inhibition 158 Luminex xMAP 484
Clinical Assessments:	None

SDY364: Systems Biology Approach to Study Influenza Vaccine 2012-13 in Healthy Children (see companion studies SDY144, SDY368, SDY387)
Status:	Updated
Description:	The treatment of pediatric immune system dysfunctions depends upon the basic understanding of its molecular and cellular components, as well as the inherent relationships between these components. Specifically, such knowledge requires an appreciation of B-Iymphocytes, T lymphocytes, natural killer cells and dendritic cells. Research investigating these cells and their functions necessitates the availability and acquisition of peripheral blood samples from healthy children to form control data groups against which various experimental conditions can be measured. Volunteers will be asked to donate blood samples to be used to further study the circulating dendritic cell subpopulations and establish their normal ranges. Blood samples will also be used to isolate antigen specific T lymphocytes, serve as a monocyte source and establish gene signatures.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3U11KLQFF
Subjects:	23
Study PI, contact:	Name Organization Site OCTAVIO RAMILO NATIONWIDE CHILDREN'S HOSPITAL NATIONWIDE CHILDREN'S HOSPITAL
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 79 Flow Cytometry 348 Hemagglutination Inhibition 92 Virus Neutralization 138
Clinical Assessments:	Medical History Vaccination History Vital Signs

SDY368: Systems Biology Approach to Study Influenza Vaccine 2013-14 in Healthy Children (see companion studies SDY364, SDY144, SDY387)
Status:	Updated
Description:	The treatment of pediatric immune system dysfunctions depends upon the basic understanding of its molecular and cellular components, as well as the inherent relationships between these components. Specifically, such knowledge requires an appreciation of B-Iymphocytes, T lymphocytes, natural killer cells and dendritic cells. Research investigating these cells and their functions necessitates the availability and acquisition of peripheral blood samples from healthy children to form control data groups against which various experimental conditions can be measured. Volunteers will be asked to donate blood samples to be used to further study the circulating dendritic cell subpopulations and establish their normal ranges. Blood samples will also be used to isolate antigen specific T lymphocytes, serve as a monocyte source and establish gene signatures.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3VUYLMJSR
Subjects:	22
Study PI, contact:	Name Organization Site OCTAVIO RAMILO NATIONWIDE CHILDREN'S HOSPITAL NATIONWIDE CHILDREN'S HOSPITAL
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 87 Flow Cytometry 352 Hemagglutination Inhibition 132 Virus Neutralization 132
Clinical Assessments:	Medical History Vaccination History Vital Signs

SDY369: Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2011/2012 Cohort (see companion studies SDY376, SDY372, SDY645)
Status:	Updated
Description:	This Project will study vaccine responses in healthy and sick children. It will address the following questions: 1) which are the best biomarkers of protective immune response to influenza vaccine in healthy children; 2) how unique autoimmune backgrounds set the stage for responsiveness/unresponsiveness to vaccines; 3) whether vaccination contributes to increase the breadth of autoimmunity in a disease-specific manner. Ultimately, we expect that these studies will shed light on basic aspects of humoral immune responses to vaccines and will permit us to discover biomarkers of response that can be applied to healthy children and to the general population.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M38SIW861C
Subjects:	4
Study PI, contact:	Name Organization Site Virginia Pascual Baylor Institute for Immunology Research Baylor Institute for Immunology Research
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 12 Flow Cytometry 100 Hemagglutination Inhibition 27 Virus Neutralization 27
Clinical Assessments:	Physical Exam

SDY372: Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2012/2013 Cohort (see companion studies (SDY369, SDY376, SDY645)
Status:	Updated
Description:	This Project will study vaccine responses in healthy and sick children. It will address the following questions: 1) which are the best biomarkers of protective immune response to influenza vaccine in healthy children; 2) how unique autoimmune backgrounds set the stage for responsiveness/unresponsiveness to vaccines; 3) whether vaccination contributes to increase the breadth of autoimmunity in a disease-specific manner. Ultimately, we expect that these studies will shed light on basic aspects of humoral immune responses to vaccines and will permit us to discover biomarkers of response that can be applied to healthy children and to the general population.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3NOD39G06
Subjects:	19
Study PI, contact:	Name Organization Site Virginia Pascual Baylor Institute for Immunology Research Baylor Institute for Immunology Research
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 71 Flow Cytometry 266 Hemagglutination Inhibition 153 Virus Neutralization 153
Clinical Assessments:	Physical Exam

SDY376: Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2013/2014 Cohort (see companion studies SDY369, SDY372, SDY645)
Status:	Updated
Description:	This Project will study vaccine responses in healthy and sick children. It will address the following questions: 1) which are the best biomarkers of protective immune response to influenza vaccine in healthy children; 2) how unique autoimmune backgrounds set the stage for responsiveness/unresponsiveness to vaccines; 3) whether vaccination contributes to increase the breadth of autoimmunity in a disease-specific manner. Ultimately, we expect that these studies will shed light on basic aspects of humoral immune responses to vaccines and will permit us to discover biomarkers of response that can be applied to healthy children and to the general population.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M37IMDD0RO
Subjects:	13
Study PI, contact:	Name Organization Site Virginia Pascual Baylor Institute for Immunology Research Baylor Institute for Immunology Research
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 47 Flow Cytometry 196 Hemagglutination Inhibition 66 Virus Neutralization 66
Clinical Assessments:	Physical Exam

SDY387: Systems Biology Approach to Study Influenza Vaccine 2010-11 in Healthy Children (see companion studies SDY144, SDY368, SDY387)
Status:	Updated
Description:	The treatment of pediatric immune system dysfunctions depends upon the basic understanding of its molecular and cellular components, as well as the inherent relationships between these components. Specifically, such knowledge requires an appreciation of B-Iymphocytes, T lymphocytes, natural killer cells and dendritic cells. Research investigating these cells and their functions necessitates the availability and acquisition of peripheral blood samples from healthy children to form control data groups against which various experimental conditions can be measured. Volunteers will be asked to donate blood samples to be used to further study the circulating dendritic cell subpopulations and establish their normal ranges. Blood samples will also be used to isolate antigen specific T lymphocytes, serve as a monocyte source and establish gene signatures.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M34N2JOQQM
Subjects:	22
Study PI, contact:	Name Organization Site Octavio Ramilo Nationwide Children's Hospital Nationwide Children's Hospital
Publications:	Induction of ICOS+CXCR3+CXCR5+ TH cells correlates with antibody responses to influenza vaccination.. Sci Transl Med. Mar 2013. doi: 10.1126/scitranslmed.3005191. [Pubmed: 23486778]
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 80 Flow Cytometry 195 Hemagglutination Inhibition 40 Virus Neutralization 120
Clinical Assessments:	Medical History Vaccination History Vital Signs

SDY465: Exploring the human maternal microbiome and its contribution to preterm birth
Status:	Updated
Description:	Preterm births occur in 12 percent of pregnancies. Preterm infants are at risk for long term health problems such as impaired hearing and vision, cerebral palsy and developmental delays. This study will characterize the human maternal microbiome and host response profiles associated with term and preterm births and identify features of each that are predicitive of preterm labor and delivery
Program/Contract:	Program Contract March of Dimes March of Dimes Human Microbiome
DOI:	10.21430/M3D491LGDT
Subjects:	47
Study PI, contact:	Name Organization Site David Relman March of Dimes Prematurity Research Center at Stanford University School of Medicine March of Dimes Prematurity Research Center at Stanford University School of Medicine
Publications:	Temporal and spatial variation of the human microbiota during pregnancy.. Proc Natl Acad Sci U S A. Sep 2015. doi: 10.1073/pnas.1502875112. Epub 2015 Aug 17. [Pubmed: 26283357]
Resources:	March of Dimes Prematurity Research Center at Stanford University http://prematurity.stanford.edu/] Stanford University R Markdown http://statweb.stanford.edu/~susan/papers/PNASRR.html.]
Assays:	Assay Type Number of Exp. Samples 16S rRNA gene sequencing 4122
Clinical Assessments:	Maternal_Health_and_Pregnancy_Status

SDY522: Differences in Antibody Responses Between Trivalent Inactivated Influenza Vaccine and Live Attenuated Influenza Vaccine (2011-12) Correlate With the Kinetics and Magnitude of Interferon Signaling in Children (see companion studies SDY144, SDY360)
Status:	Updated
Description:	The treatment of pediatric immune system dysfunctions depends upon the basic understanding of its molecular and cellular components, as well as the inherent relationships between these components. Specifically, such knowledge requires an appreciation of B-Iymphocytes, T lymphocytes, natural killer cells and dendritic cells. Research investigating these cells and their functions necessitates the availability and acquisition of peripheral blood samples from healthy children to form control data groups against which various experimental conditions can be measured. Volunteers will be asked to donate blood samples to be used to further study the circulating dendritic cell subpopulations and establish their normal ranges. Blood samples will also be used to isolate antigen specific T lymphocytes, serve as a monocyte source and establish gene signatures.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3XZMA3XL4
Subjects:	20
Study PI, contact:	Name Organization Site Octavio Ramilo Nationwide Children's Hospital Nationwide Children's Hospital
Publications:	Differences in antibody responses between trivalent inactivated influenza vaccine and live attenuated influenza vaccine correlate with the kinetics and magnitude of interferon signaling in children.. J Infect Dis. Jul 2014. doi: 10.1093/infdis/jiu079. Epub 2014 Feb 4. [Pubmed: 24495909]
Resources:
Assays:	Assay Type Number of Exp. Samples DNA microarray 72 Flow Cytometry 64 Hemagglutination Inhibition 36 Virus Neutralization 108
Clinical Assessments:	Medical History Vaccination History Vital Signs

SDY597: DC transcriptomics in response to vaccines
Status:	Updated
Description:	The mechanisms by which microbial vaccines interact with human APCs remain elusive.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3AC1WMBDO
Subjects:	20
Study PI, contact:	Name Organization Site Romain Banchereau Baylor Institute for Immunology Research Baylor Institute for Immunology Research
Publications:	Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines.. Nat Commun. Oct 2014. doi: 10.1038/ncomms6283. [Pubmed: 25335753]
Resources:	NA NA] DC Modules http://dcmodules.com/users/sign_in]
Assays:	Assay Type Number of Exp. Samples DNA microarray 413
Clinical Assessments:	None

SDY614: Mayo Clinic Smallpox Vaccine Immunogenetics Replication Study
Status:	Updated
Description:	Smallpox Vaccine Immunogenetics
Program/Contract:	Program Contract Population Genetics Analysis Program (2) Population Genetics Analysis Program: Smallpox Vaccine Immunogenetics
DOI:	10.21430/M3EEL6ILMK
Subjects:	1061
Study PI, contact:	Name Organization Site Gregory Poland Mayo Clinic Mayo Clinic
Publications:	Genome-wide association study of antibody response to smallpox vaccine.. Vaccine. Jun 2012. doi: 10.1016/j.vaccine.2012.04.055. Epub 2012 Apr 25. [Pubmed: 22542470] A large population-based association study between HLA and KIR genotypes and measles vaccine antibody responses.. PLoS One. Feb 2017. doi: 10.1371/journal.pone.0171261. eCollection 2017. [Pubmed: 28158231]
Resources:
Assays:	Assay Type Number of Exp. Samples ELISA 43744 ELISPOT 12288 HLA Typing 1056 Sequencing 108 SNP microarray 6864 Virus Neutralization 2136
Clinical Assessments:	None

SDY622: Humoral responses to Influenza vaccination in aged populations - Year 2 2012 (See companion studies SDY272 2011, SDY648 2013, SDY739 2014, SDY819 2015)
Status:	Updated
Description:	The purpose of this study was to measure the B cell responses to the Trivalent Inactivated influenza Vaccine(TIV) in young and aged subjects, and measure their different B cell subsets.
Program/Contract:	Program Contract Protective Immunity in Special Populations Defining defects in anti-viral immunity in the aged SP2 Wistar
DOI:	10.21430/M34F1R11OM
Subjects:	63
Study PI, contact:	Name Organization Site Hildegund Ertl The Wistar Institute The Wistar Institute E. John Wherry University of Pennsylvania University of Pennsylvania
Publications:	B cell responses to the 2011/12-influenza vaccine in the aged.. Aging (Albany NY). Mar 2013. doi: - [Pubmed: 23674565] Vaccine-induced boosting of influenza virus-specific CD4 T cells in younger and aged humans.. PLoS One. Oct 2013. doi: 10.1371/journal.pone.0077164. eCollection 2013. [Pubmed: 24155927] Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults.. J Immunol. Oct 2014. doi: 10.4049/jimmunol.1302503. Epub 2014 Aug 29. [Pubmed: 25172499] BTLA expression declines on B cells of the aged and is associated with low responsiveness to the trivalent influenza vaccine.. Oncotarget. Aug 2015. doi: - [Pubmed: 26277622] A shortened interval between vaccinations with the trivalent inactivated influenza vaccine increases responsiveness in the aged.. Aging (Albany NY). Dec 2015. doi: - [Pubmed: 26637961]
Resources:	H. Ertl's Laboratory http://wistar.org/lab/hildegund-cj-ertl-md]
Assays:	Assay Type Number of Exp. Samples DNA microarray 132 ELISA 1134 Flow Cytometry 1130 Virus Neutralization 378
Clinical Assessments:	None

SDY645: Systems Biology Approach to Study Influenza Vaccine in Children with Autoimmunity (Juvenile Dermatomyositis JDM) 2014/2015 Cohort (see companion studies SDY369, SDY376, SDY372)
Status:	Updated
Description:	This Project will study vaccine responses in healthy and sick children. It will address the following questions: 1) which are the best biomarkers of protective immune response to influenza vaccine in healthy children; 2) how unique autoimmune backgrounds set the stage for responsiveness/unresponsiveness to vaccines; 3) whether vaccination contributes to increase the breadth of autoimmunity in a disease-specific manner. Ultimately, we expect that these studies will shed light on basic aspects of humoral immune responses to vaccines and will permit us to discover biomarkers of response that can be applied to healthy children and to the general population.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M35HMSDTHH
Subjects:	11
Study PI, contact:	Name Organization Site Virginia Pascual Baylor Institute for Immunology Research Baylor Institute for Immunology Research
Publications:	None
Resources:	N/A N/A]
Assays:	Assay Type Number of Exp. Samples DNA microarray 39 Flow Cytometry 160 Hemagglutination Inhibition 464 Virus Neutralization 464
Clinical Assessments:	None

SDY648: Humoral responses to Influenza vaccination in aged populations - Year 3 2013 (See companion studies SDY272 2011, SDY622 2012, SDY739 2014, SDY819 2015)
Status:	Updated
Description:	The purpose of this study was to measure the B celland T cell responses to the Trivalent Inactivated influenza Vaccine(TIV) in young and aged subjects, and measure their different B cell subsets.
Program/Contract:	Program Contract Protective Immunity in Special Populations Defining defects in anti-viral immunity in the aged SP2 Wistar
DOI:	10.21430/M3E3VOEDY2
Subjects:	63
Study PI, contact:	Name Organization Site Hildegund Ertl The Wistar Institute The Wistar Institute E. John Wherry University of Pennsylvania University of Pennsylvania
Publications:	B cell responses to the 2011/12-influenza vaccine in the aged.. Aging (Albany NY). Mar 2013. doi: - [Pubmed: 23674565] Vaccine-induced boosting of influenza virus-specific CD4 T cells in younger and aged humans.. PLoS One. Oct 2013. doi: 10.1371/journal.pone.0077164. eCollection 2013. [Pubmed: 24155927] Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults.. J Immunol. Oct 2014. doi: 10.4049/jimmunol.1302503. Epub 2014 Aug 29. [Pubmed: 25172499] BTLA expression declines on B cells of the aged and is associated with low responsiveness to the trivalent influenza vaccine.. Oncotarget. Aug 2015. doi: - [Pubmed: 26277622] A shortened interval between vaccinations with the trivalent inactivated influenza vaccine increases responsiveness in the aged.. Aging (Albany NY). Dec 2015. doi: - [Pubmed: 26637961]
Resources:	H. Ertl's Laboratory http://wistar.org/lab/hildegund-cj-ertl-md]
Assays:	Assay Type Number of Exp. Samples DNA microarray 124 ELISA 1134 Flow Cytometry 1130 Virus Neutralization 378
Clinical Assessments:	None

SDY667: The immune signature of palmoplantar pustulosis
Status:	Updated
Description:	This study will compare adult patients with CPP(chronic plaque type psoriasis ), who are currently not undergoing topical, systemic or biologic treatment and matched healthy controls. Baseline demographics and psoriasis history will be recorded. All PPP patients will be comprehensively phenotyped and psoriasis severity assessed including the body surface area (BSA) involved, Psoriasis Area and Severity Index (PASI), Physicians Global assessment (PGA) and Dermatology Life Quality Index (DLQI).
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M34NBAVDGJ
Subjects:	50
Study PI, contact:	Name Organization Site Gerlinde Obermoser Baylor Institute for Immunology research Baylor Institute for Immunology research
Publications:	None
Resources:	N/A N/A]
Assays:	Assay Type Number of Exp. Samples DNA microarray 138
Clinical Assessments:	None

SDY670: Molecular Profiling of Donor and Recipient Proinflammatory and Allograft Response (CTOT-03)
Status:	Updated
Description:	Inflammation and injuries to transplanted organs during the immediate post-operative period may be linked to early organ dysfunction and higher rates of transplant rejection in the recipient. Currently, mRNA expression of proinflammatory genes in donor tissues is thought to be a risk factor for early organ transplant dysfunction, increased expression of the recipients cell-mediated immunity genes, and organ rejection. The purpose of this study is to test the association between proinflammatory mRNA expression in donor samples and subsequent development of early organ dysfunction in kidney, lung, and liver transplant recipients. This study will also test the effects of proinflammatory mediators expressed in the transplanted organ pre- and post-reperfusion on organ rejection and genes expressed in cell mediated immune responses. This will be achieved by identifying the proinflammatory immune responses and their mechanisms.
Program/Contract:	Program Contract Clinical Trials in Organ Transplantation (CTOT) MOLECULAR PROFILING AND IMMUNOMODULATORY INTERVENTIONS (CTOT-03, CTOT-04)
DOI:	10.21430/M3GLG1R2NY
Subjects:	311
Study PI, contact:	Name Organization Site Abraham Shaked University of Pennsylvania Medical Center University of Pennsylvania Medical Center
Publications:	Validation of a current definition of early allograft dysfunction in liver transplant recipients and analysis of risk factors.. Liver Transpl. Aug 2010. doi: 10.1002/lt.22091. [Pubmed: 20677285] Gene set enrichment analysis identifies key innate immune pathways in primary graft dysfunction after lung transplantation.. Am J Transplant. Jul 2013. doi: 10.1111/ajt.12283. Epub 2013 May 24. [Pubmed: 23710539] Peripheral Blood Gene Expression Changes Associated With Primary Graft Dysfunction After Lung Transplantation.. Am J Transplant. Jul 2017. doi: 10.1111/ajt.14209. Epub 2017 Feb 17. [Pubmed: 28117940]
Resources:	ClinicalTrials.gov https://www.clinicaltrials.gov/ct/show/NCT00531921] NIH RePORTER http://projectreporter.nih.gov/project_info_description.cfm?aid=6875931&icde=25772230]
Assays:	Assay Type Number of Exp. Samples DNA microarray 286 HLA Typing 495
Clinical Assessments:	Biopsy Echocardiogram Electrocardiogram Pathology Rejection

SDY673: Characterization of maternal serum gene expression and microbiome
Status:	Updated
Description:	Plasma cell-free RNA (cfRNA) encompasses a broad spectrum of RNA species that can be derived from both human cells and microbes. Because cfRNA is fragmented and of low concentration, it has been challenging to profile its transcriptome using standard RNA-seq methods. This study assessed several recent RNA-seq methods on cfRNA samples to analyze the dynamic changes of both the human transcriptome and the microbiome of plasma during pregnancy. cfRNA reflected a well-orchestrated immune modulation during pregnancy: an up-regulation of anti-inflammatory genes and an increased abundance of anti-microbial genes, while the plasma microbiome remained relatively stable during pregnancy. The bacteria Ureaplasma shows an increased prevalence and increased abundance at postpartum, which is likely to be associated with postpartum infection. This study demonstrated that cfRNA-seq can be used to detect a number of human pathogens, including high loads of human parvovirus B19 virus (B19V), known to be a potential cause of complications in pregnancy.
Program/Contract:	Program Contract March of Dimes March of Dimes Human Microbiome
DOI:	10.21430/M3OARGGSY0
Subjects:	50
Study PI, contact:	Name Organization Site Stephen Quake March of Dimes Prematurity Research Center at Stanford University School of Medicine March of Dimes Prematurity Research Center at Stanford University School of Medicine
Publications:	Simultaneously Monitoring Immune Response and Microbial Infections During Pregnancy through Plasma cfRNA Sequencing.. Clin Chem. Sep 2017. doi: - [Pubmed: 28904056]
Resources:	MOD_Stanford_Prematurity_Center http://prematurity.stanford.edu/] SRA https://www.ncbi.nlm.nih.gov/bioproject/PRJNA400333/]
Assays:	Assay Type Number of Exp. Samples Q-PCR 111 RNA sequencing 130 Sequencing 58
Clinical Assessments:	None

SDY690: Systems Biology Study to Investigate Immune Correlates to Hepatitis B Vaccine Engerix-B comparing cellular responses and gene expression patterns between PBMCs (cell sorts) and whole blood samples (see companion studies SDY816 and SDY299)
Status:	Updated
Description:	This project will contribute to the overall vision and goals of this U19 by analyzing the role of adjuvants in the humoral response to hep B vaccination in healthy individuals.
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3F8W7VA7O
Subjects:	12
Study PI, contact:	Name Organization Site Robert Coffman Dynavax Technologies Corporation Dynavax Technologies Corporation
Publications:	Demonstration of safety and enhanced seroprotection against hepatitis B with investigational HBsAg-1018 ISS vaccine compared to a licensed hepatitis B vaccine.. Vaccine. Mar 2012. doi: 10.1016/j.vaccine.2012.02.001. Epub 2012 Feb 14. [Pubmed: 22342916] Immunogenicity and safety of an investigational hepatitis B vaccine with a Toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared to a licensed hepatitis B vaccine in healthy adults 40-70 years of age.. Vaccine. Nov 2013. doi: 10.1016/j.vaccine.2013.05.068. Epub 2013 May 30. [Pubmed: 23727002] Immunogenicity of an investigational hepatitis B vaccine with a toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared with a licensed hepatitis B vaccine in subpopulations of healthy adults 18-70 years of age.. Vaccine. Jul 2015. doi: 10.1016/j.vaccine.2015.05.070. Epub 2015 Jun 9. [Pubmed: 26067185] None. None None None. doi: None [Pubmed: 29289383]
Resources:	NA NA]
Assays:	Assay Type Number of Exp. Samples DNA microarray 192
Clinical Assessments:	None

SDY739: Humoral responses to Influenza vaccination in aged populations - Year 4 2014 (See companion studies SDY272 2011, SDY622 2012, SDY648 2013, SDY819 2015)
Status:	Updated
Description:	The purpose of this study was to measure the B celland T cell responses to the Trivalent Inactivated influenza Vaccine(TIV) in young and aged subjects, and measure their different B cell subsets.
Program/Contract:	Program Contract Protective Immunity in Special Populations Defining defects in anti-viral immunity in the aged SP2 Wistar
DOI:	10.21430/M352VU0YNY
Subjects:	65
Study PI, contact:	Name Organization Site Hildegund Ertl The Wistar Institute The Wistar Institute
Publications:	B cell responses to the 2011/12-influenza vaccine in the aged.. Aging (Albany NY). Mar 2013. doi: - [Pubmed: 23674565] Vaccine-induced boosting of influenza virus-specific CD4 T cells in younger and aged humans.. PLoS One. Oct 2013. doi: 10.1371/journal.pone.0077164. eCollection 2013. [Pubmed: 24155927] Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults.. J Immunol. Oct 2014. doi: 10.4049/jimmunol.1302503. Epub 2014 Aug 29. [Pubmed: 25172499] BTLA expression declines on B cells of the aged and is associated with low responsiveness to the trivalent influenza vaccine.. Oncotarget. Aug 2015. doi: - [Pubmed: 26277622] A shortened interval between vaccinations with the trivalent inactivated influenza vaccine increases responsiveness in the aged.. Aging (Albany NY). Dec 2015. doi: - [Pubmed: 26637961]
Resources:	H. Ertl's Laboratory http://wistar.org/lab/hildegund-cj-ertl-md]
Assays:	Assay Type Number of Exp. Samples DNA microarray 126 ELISA 1134 Flow Cytometry 498 Virus Neutralization 378
Clinical Assessments:	None

SDY753: Investigating Alterations to the Nasal Microbiome after Vaccination with LAIV
Status:	Updated
Description:	The goal of this proposal is to characterize alterations to the nasal microbiome after vaccination with the 2012-2013 seasonal LAIV and to correlate these changes with LAIV-specific immune responses (A/California/7/2009 (H1N1) and A/Victoria/361/2011 (H3N2)).
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Bioinformatics Approach to Influenza A/H1N1 Vaccine Immune Profiling
DOI:	10.21430/M3ENN2D3SZ
Subjects:	47
Study PI, contact:	Name Organization Site Gregory Poland Mayo Clinic Mayo Clinic
Publications:	None
Resources:
Assays:	Assay Type Number of Exp. Samples ELISA 94 Sequencing 121
Clinical Assessments:	None

SDY775: Evaluating differences between healthy placental microbiome and contamination control sample microbiomes
Status:	Updated
Description:	Placental samples from healthy deliveries were compared to an extensive set of bacterial contamination controls as well as to oral and vaginal samples from the same women. This control study was unable to distinguish bacterial species and abundance between placental and contamination control samples
Program/Contract:	Program Contract March of Dimes March of Dimes Human Microbiome
DOI:	10.21430/M3PZM1ERD2
Subjects:	7
Study PI, contact:	Name Organization Site Frederic Bushman University of Pennsylvania School of Medicine University of Pennsylvania School of Medicine
Publications:	Comparison of placenta samples with contamination controls does not provide evidence for a distinct placenta microbiota.. Microbiome. Jun 2016. doi: 10.1186/s40168-016-0172-3. [Pubmed: 27338728]
Resources:
Assays:	Assay Type Number of Exp. Samples 16S rRNA gene sequencing 69 Q-PCR 69
Clinical Assessments:	None

SDY816: Systems Biology Analysis of the response to Licensed Hepatitis B Vaccine (HEPLISAV) in specific cell subsets (see companion studies SDY299 and SDY690)
Status:	Updated
Description:	None
Program/Contract:	Program Contract Human Immunology Project Consortium 1 (HIPC1) Systems Analysis Vaccine Responses in Healthy and Hyporesponsive Humans
DOI:	10.21430/M3HV9NRS67
Subjects:	10
Study PI, contact:	Name Organization Site Robert Coffman Dynavax Technologies Corporation Dynavax Technologies Corporation
Publications:	Demonstration of safety and enhanced seroprotection against hepatitis B with investigational HBsAg-1018 ISS vaccine compared to a licensed hepatitis B vaccine.. Vaccine. Mar 2012. doi: 10.1016/j.vaccine.2012.02.001. Epub 2012 Feb 14. [Pubmed: 22342916] Immunogenicity and safety of an investigational hepatitis B vaccine with a Toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared to a licensed hepatitis B vaccine in healthy adults 40-70 years of age.. Vaccine. Nov 2013. doi: 10.1016/j.vaccine.2013.05.068. Epub 2013 May 30. [Pubmed: 23727002] Immunogenicity of an investigational hepatitis B vaccine with a toll-like receptor 9 agonist adjuvant (HBsAg-1018) compared with a licensed hepatitis B vaccine in subpopulations of healthy adults 18-70 years of age.. Vaccine. Jul 2015. doi: 10.1016/j.vaccine.2015.05.070. Epub 2015 Jun 9. [Pubmed: 26067185] None. None None None. doi: None [Pubmed: 29289383]
Resources:	NA NA]
Assays:	Assay Type Number of Exp. Samples DNA microarray 509
Clinical Assessments:	None

SDY839: Measles Immunity
Status:	Updated
Description:	None
Program/Contract:	Program Contract Immunogenetics of Measles Immunity Immunogenetics of Measles Immunity
DOI:	10.21430/M3XWLPC8A2
Subjects:	2681
Study PI, contact:	Name Organization Site Gregory Poland Mayo Clinic Mayo Clinic
Publications:	A large population-based association study between HLA and KIR genotypes and measles vaccine antibody responses.. PLoS One. Feb 2017. doi: 10.1371/journal.pone.0171261. eCollection 2017. [Pubmed: 28158231]
Resources:
Assays:	Assay Type Number of Exp. Samples ELISPOT 14598 Sequencing 54 Virus Neutralization 2626
Clinical Assessments:	None

SDY1164: Vaginal microbial signature for preterm birth
Status:	Updated
Description:	Recent studies about association between maternal vaginal microbiota and risks for preterm birth (PTB) conflicted along similar lines: Caucasian and Asian women cohorts showed associations between PTB and low Lactobacillus vaginal communities (BV-like) while no significant association with PTB was detected in cohorts of African-American women. This study compares two new larger cohorts of women at low and high risk for PTB, addressing two challenges: (i) low power resulting from the combination of small study populations (30?91 pregnant women), the many taxa measured by metabarcoding, and the absence of initial hypotheses more specific than some difference between preterm and term gestations; and (ii) insufficient understanding of population-specific factors that might modulate the PTB?microbiota association.
Program/Contract:	Program Contract March of Dimes March of Dimes Human Microbiome
DOI:	10.21430/M37W3869AH
Subjects:	136
Study PI, contact:	Name Organization Site David Relman March of Dimes Prematurity Research Center at Stanford University School of Medicine March of Dimes Prematurity Research Center at Stanford University School of Medicine
Publications:	Replication and refinement of a vaginal microbial signature of preterm birth in two racially distinct cohorts of US women.. Proc Natl Acad Sci U S A. Aug 2017. doi: - [Pubmed: 28847941]
Resources:	March of Dimes Prematurity Research Center at Stanford University http://prematurity.stanford.edu/] SRA ? Raw sequencing data https://www.ncbi.nlm.nih.gov/sra/SRP115697] Stanford Digital Repository - Sequence table and R scripts for analysis workflow https://purl.stanford.edu/yb681vm1809]
Assays:	Assay Type Number of Exp. Samples 16S rRNA gene sequencing 2367
Clinical Assessments:	Pregnancy Delivery

SDY1175: Human microbiome characterization through massive shotgun sequencing of circulating cell-free DNA.
Status:	Updated
Description:	Cell-free DNA-derived microbiomes of 1,351 samples from 188 patients in four longitudinally sampled cohorts were analyzed. The majority of assembled sequences from cfDNA are derived from previously unidentified organisms, for instance numerous novel anelloviruses in immunocompromised patients, which represent a doubling of identified members in that viral family. Over two thirds of the sequences are bacterial, and the majority are most similar to proteobacteria; however, many large contigs can only be classified at the phylum or superkingdom level. Numerous novel phages were also found throughout the population. Multiple independent analyses confirm the existence of these novel sequences.
Program/Contract:	Program Contract March of Dimes March of Dimes Human Microbiome
DOI:	10.21430/M33PSZ2FHV
Subjects:	16
Study PI, contact:	Name Organization Site Stephen Quake March of Dimes Prematurity Research Center at Stanford University School of Medicine March of Dimes Prematurity Research Center at Stanford University School of Medicine
Publications:	Numerous uncharacterized and highly divergent microbes which colonize humans are revealed by circulating cell-free DNA.. Proc Natl Acad Sci U S A. Sep 2017. doi: 10.1073/pnas.1707009114. Epub 2017 Aug 22. [Pubmed: 28830999]
Resources:	March of Dimes Prematurity Research Center at Stanford University http://prematurity.stanford.edu/] SRA ? Lung transplant sequencing data https://www.ncbi.nlm.nih.gov/sra/PRJNA263522] SRA ? Bone marrow transplant sequencing data https://www.ncbi.nlm.nih.gov/sra/PRJNA385009] SRA ? Heart transplant sequencing data https://www.ncbi.nlm.nih.gov/sra/PRJNA222186] SRA - Pregnancy sequencing data https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA385180]
Assays:	Assay Type Number of Exp. Samples Sequencing 58
Clinical Assessments:	None

SDY1206: Early pregnancy vaginal microbiome trends and preterm birth (see companion study SDY1341)
Status:	Updated
Description:	Previous studies have shown that vaginal microbiota of asymptomatic, nonpregnant, reproductive age women cluster into 5 distinct ?community-state types?, which differ both by dominant Lactobacillus species as well as overall community composition. A much greater proportion of African-American and Hispanic women harbored a non-Lactobacillus-dominant community, suggesting that in some women a non-Lactobacillus-based vaginal community may be a normal variant. A recent study examined vaginal microbial composition and the risk for preterm birth but had very few African-American subjects and few preterm births. This study characterizes vaginal microbial community characteristics over time in a large predominantly African-American cohort of pregnant women and test whether particular community characteristics are associated with the risk for subsequent preterm birth.
Program/Contract:	Program Contract March of Dimes March of Dimes Human Microbiome
DOI:	10.21430/M3H1U3KJMZ
Subjects:	77
Study PI, contact:	Name Organization Site Methodius Tuuli Washington University in St Louis School of Medicine, St. Louis, MO Washington University in St Louis School of Medicine, St. Louis, MO Molly Stout Washington University in St Louis School of Medicine, St. Louis, MO Washington University in St Louis School of Medicine, St. Louis, MO
Publications:	Early pregnancy vaginal microbiome trends and preterm birth.. Am J Obstet Gynecol. Sep 2017. doi: 10.1016/j.ajog.2017.05.030. Epub 2017 May 23. [Pubmed: 28549981]
Resources:	SRA ? Microbiome Data https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294119/]
Assays:	Assay Type Number of Exp. Samples 16S rRNA gene sequencing 149
Clinical Assessments:	Pregnancy History