| dead |
Dead cell stains are membrane-impermeant dyes that are excluded by viable cells, but stain necrotic or late apoptotic cells with damaged plasma membranes. |
https://www.tcd.ie/ttmi/facilities/fcf/assets/pdf/dead-cell-stains.pdf |
| granulocyte |
A leukocyte with abundant granules in the cytoplasm. Granulocye gate is often set on FSC vs SSC plot position. |
http://purl.obolibrary.org/obo/CL_0000094 |
| infected |
Infection marker for HIPC Standard for specifying cell populations |
https://www.ncbi.nlm.nih.gov/books/NBK26833/ |
| intact |
Intact gate is often set on FSC vs SSC plot position. |
https://www.biology-online.org/dictionary/Intact |
| leukocyte |
A type of blood cell that is made in the bone marrow and found in the blood and lymph tissue. Leukocytes are part of the body's immune system. They help the body fight infection and other diseases. Types of leukocytes are granulocytes (neutrophils, eosinophils, and basophils), monocytes, and lymphocytes (T cells and B cells). Checking the number of leukocytes in the blood is usually part of a complete blood cell (CBC) test. It may be used to look for conditions such as infection, inflammation, allergies, and leukemia. Also called WBC and white blood cell. |
https://www.cancer.gov/publications/dictionaries/cancer-terms/def/leukocyte |
| lymphocyte |
A lymphocyte is a leukocyte commonly found in the blood and lymph that has the characteristics of a large nucleus, a neutral staining cytoplasm, and prominent heterochromatin. Lymphocyte gate is often set on FSC vs SSC plot position. |
http://purl.obolibrary.org/obo/CL_0000542 |
| MHC:epitope |
MHC:epitope staining for HIPC Standard for specifying cell populations |
https://en.wikipedia.org/wiki/Epitope |
| monocyte |
Myeloid mononuclear recirculating leukocyte that can act as a precursor of tissue macrophages, osteoclasts and some populations of tissue dendritic cells. Monocyte gate is often set on FSC vs SSC plot position. |
http://purl.obolibrary.org/obo/CL_0000576 |
| proliferated |
dye |
|
| singlet |
When Area Scaling is set properly, the value of A = H for a singlet event. |
https://www.researchgate.net/post/How_does_FSC-A_vs_FSC-H_indicate_the_singlets_in_a_FACS_data |
| SSC |
The light scatter is measured by two optical detectors. One detector measures scatter along the path of the laser. This parameter is referred to as forward scatter (FSC). The other detector measures scatter at a ninety degree angle relative to the laser. This parameter is called side scatter (SSC). When measured in conjunction, these two measurements allow for some degree of cellular differentiation within a heterogeneous population. |
https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-with-flowjo/58 |
| viable |
In flow cytometry, live cells are distinguished from dead cells or debris by several measures. The non-debris gate is often set on FSC vs SSC plot position. Propidium iodide and Annexin V are commonly used to detect apoptotic cells by their binding to cellular components that are intracellular in viable cells. |
https://en.wikipedia.org/wiki/Annexin_A5
https://en.wikipedia.org/wiki/Propidium_iodide
|