Lk_Reagent_Type

Controlled vocabulary for Reagent Type.

Columns
Name Type Description Foreign Key
name varchar(50) Name or identifier
description varchar(1000) Free text to expand upon details
link varchar(2000) Link to URL
Indexes
Name Column Description
PRIMARY name BTREE
Tables that Reference this Table
Name Column Table Reference Column Reference
reagent type lk_reagent_type name
Values
name description link
Array Arrays (including microarrays) are a set of probes immobilized on a surface. The probes can be oligonucleotides, cDNAs, antibodies and other molecules that recognize a target. Microarrays can be constructed by several methods including (but not limited to in situ oligo synthesis (e.g. Affymetrix), cDNA spotting, bead arrays (e.g. Illumina) and antibody spotting. The position and identity of probes are provided by the manufacturer. The probe identifiers and their target are referred to as annotation or translation of probe identifiers to bioinformatic identifiers. Microarrays can be used for gene expression (mRNA transcript quantification), genotyping, cytokine quantification, etc. Microarrays for gene expression fall into two general classes- single channel and dual channel. The channel refers to the wavelength scanned for fluorescent signals. Affymetrix microarrays are obligatory single channel. Many commercial and non-commercial microarray manufacturers use two channel. http://purl.obolibrary.org/obo/OBI_0400147
http://purl.obolibrary.org/obo/OBI_0001204
http://purl.obolibrary.org/obo/OBI_0001307
http://purl.obolibrary.org/obo/OBI_0400149
CyTOF Cytometry Time Of Flight CyTOF (DVS Sciences) or Mass cytometry, or , is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Readout is by time-of-flight mass spectrometry. http://en.wikipedia.org/wiki/Mass_cytometry
Cytometric Bead Array An assay in which a series of beads coated with antibodies specific for different analytes and marked with discrete fluorescent labels are used to simultaneously capture and quantitate soluble analytes using flow cytometric analysis. http://purl.obolibrary.org/obo/OBI_0000920
ELISA Enzyme-Linked ImmunoSorbant Assay. Quantification of a molecule (e.g cytokine) by an antibody immobilization strategy. http://purl.obolibrary.org/obo/OBI_0000661
ELISPOT Enzyme-linked ImmunoSPOT. A variant of ELISA with increased resolution that allows quantifying the number of cells in a population that release a molecule (e.g. cytokine). http://purl.obolibrary.org/obo/OBI_0600031
Flow Cytometry Fluorescence Activated Cell Sorting. http://purl.obolibrary.org/obo/OBI_0000916
Hemagglutination Inhibition Quantitate serum antibody to a specific antigen by blocking agglutination of cells. http://purl.obolibrary.org/obo/OBI_0000875
HLA Typing Human Leukocyte Antigen typing. http://purl.obolibrary.org/obo/OBI_0000435
KIR Typing Killer cell immunoglobulin-like receptors. http://purl.obolibrary.org/obo/OBI_0000435
Luminex xMAP Microsphere based multiplexing system. Microspheres are color coded and linked to a detector or capture reagent (e.g. antibody, oligonucleotides, peptides, or receptors). http://purl.obolibrary.org/obo/OBI_0000920
Neutralizing Antibody Titer Measurement of how much antibody an organism has produced that recognizes a particular epitope, expressed as the inverse of the greatest dilution that still gives a positive result. http://purl.obolibrary.org/obo/VO_0000397
Other Other reagent. http://immport.org/immport-open/public/home/documentation
PCR Polymerase Chain Reaction is a technique to amplify a DNA template. http://purl.obolibrary.org/obo/OBI_0000415
Sequencing Sequencing is used to discover new sequence variants and to genotype a sample for known variants. http://purl.obolibrary.org/obo/OBI_0600047
Virus Neutralization Block a viral function. http://purl.obolibrary.org/obo/OBI_0000872